Product Description
HiScript IV RT SuperMix for qPCR (+gDNA wiper) is an upgraded version of HiScript III RT SuperMix for qPCR (+gDNA wiper), including a new generation of HiScript IV Reverse Transcriptase and Buffer optimized for reverse transcription. This kit further improves the synthesis efficiency of cDNA, making it a better choice for reverse transcription of low-input, low-expression or degraded RNA templates. 5 × gDNA wiper Mix can completely remove the genomic contaimination in the RNA template, so there is no need to design intron-spanning qPCR primers. 4 × HiScript IV qRT SuperMix contains all the components required for the reverse transcription, just add template RNA and RNase-free ddH2O to start the reaction.
Features
Superior reverse transcription efficiency: Achieve qPCR detection results with smaller CT values.
Great gDNA elimination efficiency: Thoroughly remove 500 ng of gDNA to ensure the accuracy of qPCR detection.
Excellent template compatibility: Efficient reverse transcription of low-input, low-expression or degraded RNA templates.
Components
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
Performance
Faster Reaction
Superior RT Efficiency
100 ng RNA from animals, plants and microbes were reverse transcribed by Vazyme #R423 and similar RT products from Supplier A, Supplier B and Supplier C, following their respective manuals. The RT efficiency of these reagents was evaluated by qPCR amplification of obtained cDNA. It can be seen from the figure, △CT between Vazyme #R423 and Supplier C is within 0.5, indicating a similar efficiency of them; △CT between Vazyme #R423 and Suppler A/Supplier B are more than 0.5, indicating a better efficiency of Vazyme #R423.
Great gDNA Elimination Efficiency
500 ng gDNA from 293T cells were eliminated by Vazyme #R423 and similar RT products from Supplier A, Supplier B and Supplier C. The digested gDNA were amplified by qPCR to evaluate the elimination efficiency of these products. The results show that the gDNA elimination efficiency of Vazyme #R423 is consistent with that of Supplier A and Supplier B, and significantly better than that of Supplier C.
Excellent Template Compatibility
100 ng human FFPE samples and degraded 293T cells (Rin=1) were reverse transcribed by Vazyme #R423 and similar RT products from Supplier A, Supplier B and Supplier C. The template compatibility of these reagents was evaluated by qPCR amplification of obtained cDNA. The results show that △CT between Vazyme #R423 and Suppliers are more than 0.5 in most genes, indicating a better template compatibility of Vazyme #R423.
Selection Guide
Vazyme Reverse Transcription Kit Selection Guide | ||||
Universal RT kit (Primers are available individually packaged) | RT-qPCR SuperMix (Specially designed for qPCR) | All-in-one RT-qPCR SuperMix (Minimum Setup Time) | All-in-one RT-qPCR SuperMix with red dye (Color tracing) | |
Product | HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper) Best | HiScript IV RT SuperMix for qPCR (+gDNA wiper) Popular | HiScript IV All-in-One Ultra RT SuperMix for qPCR Fast | HiScript IV All-in-One RT Red SuperMix for qPCR (+gDNA Wiper)Color |
Cat.No. | #R412 | #R423 | #R433 | #RT433 |
Format | 3 tubes | 1 tube | 1 tube | 1 tube |
Kit contents | 5 × gDNA wiper Mix 4 × HiScript IV RT SuperMix Oligo (dT)20VN Random Primers 4 × No RT Control Mix RNase-free ddH2O | 5 × gDNA wiper Mix 4 × HiScript IV qRT SuperMix 4 × No RT Control Mix RNase-free ddH2O | 4 × All-in-one Ultra qRT SuperMix RNase-free ddH2O | 4 × HiScript IV All-in-One qRT Red SuperMix RNase-free ddH2O |
Optimal reaction temperature | PCR: 50℃ qPCR: 37℃-50℃ | 37℃-50℃ | 50℃ | 50℃ |
Application | Flexibility in reaction conditions | First-strand cDNA synthesis reaction mix for two step RT-qPCR | Complete gDNA elimination and Reverse transcription in only 1 step | For customers who subsequently use 384-well plates for qPCR or require color-based tracing. |
