Product Description
HiScript IV All-in-One Ultra RT SuperMix for qPCR (+gDNA wiper) (R433) is a next-generation reverse transcription supermix designed for reverse transcription for qPCR, enabling faster and simpler cDNA synthesis.
Powered by Vazyme’s innovative All-in-One technology, genomic DNA removal and reverse transcription are integrated into a single reaction, eliminating the need for separate DNase treatment or additional reagent handling. The optimized system completes reverse transcription in as little as 5 minutes, enabling a streamlined one-tube, one-reaction workflow from RNA to qPCR-ready cDNA.Featuring the high-performance HiScript IV Reverse Transcriptase, R433 delivers efficient cDNA synthesis across a broad range of RNA templates, including low-input RNA and challenging templates with complex secondary structures. Combined with effective gDNA removal and a simplified workflow, R433 provides a fast, convenient, and reliable solution for routine and high-throughput gene expression analysis.
Features
True All-in-One Workflow
Integrates gDNA removal and reverse transcription in a single reaction, eliminating separate DNase treatment and simplifying the traditional multi-step RT workflow.
Ultra-Fast 5-Minute Reverse Transcription
Complete reverse transcription in as little as 5 minutes, significantly reducing turnaround time from RNA to qPCR-ready cDNA.
Efficient gDNA Removal
The integrated gDNA removal system effectively minimizes genomic DNA contamination, helping improve the reliability of downstream gene expression analysis.
High-Efficiency cDNA Synthesis
Powered by HiScript IV Reverse Transcriptase with high RNA affinity for efficient and consistent cDNA synthesis across a wide range of RNA inputs.
Excellent Performance with Challenging Templates
Optimized for difficult RNA templates, including GC-rich regions and complex secondary structures, for robust reverse transcription performance.
Built for RT-qPCR
Optimized for downstream real-time PCR applications, providing a streamlined RNA → cDNA → qPCR workflow for routine and high-throughput gene expression studies.
Components
Storage
Storage: -30°C to -15°C
Shipping: Transport at ≤0°C
Performance
Faster Reaction
Complete genomic DNA removal and reverse transcription in 5 minutes.
Total RNA from 293T cells (100 ng) was used as a template for reverse transcription reactions with Vazyme #R433, following the manufacturer’ s protocol at different reverse transcription times (5 min, 10 min, and 15 min). The resulting cDNA was then subjected to qPCR quantification for multiple genes using Vazyme #Q712. The results demonstrate that Vazyme #R433 achieved effective reverse transcription at both the 5-minute rapid time and the 15-minute standard time.
Higher Reverse Transcription Efficiency
Reverse transcription reactions were conducted using 100 ng of RNA from animal cells, plant tissues, and animal tissues as templates with Vazyme #R433, Vazyme #R333, and other commercially available reverse transcription reagents (Supplier A and B), following the recommended protocols for each product. The resulting cDNA underwent quantitative analysis of multiple genes through qPCR. The results indicate that Vazyme #R433 consistently yielded lower CT values across different testing systems, indicating superior reverse transcription efficiency compared to both Vazyme #R333 and the products from Supplier A and B.
Applications
Gene Expression Analysis
For accurate relative quantification of target genes.
High-Throughput Gene Expression Analysis
Ideal for rapid processing of large numbers of RNA samples.
Low-Abundance Transcript Detection
Suitable for sensitive detection of low-abundance RNA targets.
gDNA-Sensitive Applications
Ideal for gene expression assays where genomic DNA contamination may affect quantification accuracy.
Challenging RNA Templates
Suitable for GC-rich transcripts and RNA templates with complex secondary structures.
Broad RNA Sample Compatibility
Compatible with RNA from animal, plant, and cultured cell samples.
Selection Guide
Vazyme Reverse Transcription Kit Selection Guide | ||||
Universal RT kit (Primers are available individually packaged) | RT-qPCR SuperMix (Specially designed for qPCR) | All-in-one RT-qPCR SuperMix (Minimum Setup Time) | All-in-one RT-qPCR SuperMix with red dye (Color tracing) | |
Product | HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper) Best | HiScript IV RT SuperMix for qPCR (+gDNA wiper) Popular | HiScript IV All-in-One Ultra RT SuperMix for qPCR Fast | HiScript IV All-in-One RT Red SuperMix for qPCR (+gDNA Wiper)Color |
Cat.No. | #R412 | #R423 | #R433 | #RT433 |
Format | 3 tubes | 1 tube | 1 tube | 1 tube |
Kit contents | 5 × gDNA wiper Mix 4 × HiScript IV RT SuperMix Oligo (dT)20VN Random Primers 4 × No RT Control Mix RNase-free ddH2O | 5 × gDNA wiper Mix 4 × HiScript IV qRT SuperMix 4 × No RT Control Mix RNase-free ddH2O | 4 × All-in-one Ultra qRT SuperMix RNase-free ddH2O | 4 × HiScript IV All-in-One qRT Red SuperMix RNase-free ddH2O |
Optimal reaction temperature | PCR: 50℃ qPCR: 37℃-50℃ | 37℃-50℃ | 50℃ | 50℃ |
Application | Flexibility in reaction conditions | First-strand cDNA synthesis reaction mix for two step RT-qPCR | Complete gDNA elimination and Reverse transcription in only 1 step | For customers who subsequently use 384-well plates for qPCR or require color-based tracing. |
FAQS
Frequently Asked Questions1. What makes R433 different from conventional reverse transcription kits?R433 features an innovative All-in-One workflow that integrates genomic DNA removal and reverse transcription in a single reaction. No separate DNase treatment or intermediate reagent addition is required, making the workflow faster and simpler.
2. How fast is the reverse transcription reaction?R433 can complete reverse transcription in as little as 5 minutes, making it ideal for rapid and high-throughput gene expression workflows.
3. Does R433 require a separate genomic DNA removal step?No. The gDNA removal and reverse transcription processes are integrated into one reaction, eliminating the need for a separate DNase treatment step.
4. Do I need to open the tube or add reagents between gDNA removal and reverse transcription?No. R433 is designed as a true one-tube All-in-One system. Once the reaction is prepared, no intermediate tube opening or additional reagent handling is required.
5. What applications is R433 recommended for?R433 is designed for cDNA synthesis followed by RT-qPCR-based gene expression analysis, including routine gene expression studies, high-throughput analysis, low-abundance transcript detection, and challenging RNA templates.
6. Can R433 be used with challenging RNA templates?Yes. The HiScript IV Reverse Transcriptase provides high RNA affinity and robust reverse transcription performance, making R433 suitable for challenging templates such as GC-rich RNA and transcripts with complex secondary structures.
7. What types of RNA samples are compatible with R433?R433 is compatible with a broad range of RNA samples, including RNA derived from animal tissues, plant samples, and cultured cells.
8. Can the synthesized cDNA be used directly for qPCR?Yes. The cDNA generated with R433 is optimized for downstream qPCR applications. Follow the recommended cDNA input guidelines when setting up the qPCR reaction.
9. Is R433 suitable for high-throughput experiments?Yes. By combining gDNA removal and reverse transcription into a single reaction and completing RT in as little as 5 minutes, R433 reduces hands-on steps and overall processing time, making it particularly suitable for high-throughput workflows.
10. Does R433 support both oligo(dT) and random priming?Yes. R433 uses an optimized primer system to support efficient reverse transcription of RNA templates for downstream gene expression analysis.
