Product Description
HiScript IV All-in-One RT Red SuperMix for qPCR (+gDNA Wiper) is an upgraded all-in-one reverse transcription reagent designed for qPCR applications. It integrates all necessary components for genomic DNA removal and RNA reverse transcription into a single-tube formulation, requiring only the addition of template RNA and ddH₂O to initiate the reaction. Pipetting can be visually confirmed during cDNA synthesis. The optimized red tracking dye, with no interference in fluorescent signal acquisition for downstream dye- or probe-based qPCR assays, ensures amplification sensitivity and plateau-phase stability.
Features
Visual pipetting: Easy sample addition without visual strain, reducing the probability of incorrect or missed addition during pipetting
User-friendly operation: “One tube” reagent “one-step” completes genome removal & reverse transcription, accelerating research progress
Highly effective reverse transcription: Achieves lower CT values compared to commercially available products of the same type
Components
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
Selection Guide
Vazyme Reverse Transcription Kit Selection Guide | ||||
Universal RT kit (Primers are available individually packaged) | RT-qPCR SuperMix (Specially designed for qPCR) | All-in-one RT-qPCR SuperMix (Minimum Setup Time) | All-in-one RT-qPCR SuperMix with red dye (Color tracing) | |
Product | HiScript IV 1st Strand cDNA Synthesis Kit (+gDNA wiper) Best | HiScript IV RT SuperMix for qPCR (+gDNA wiper) Popular | HiScript IV All-in-One Ultra RT SuperMix for qPCR Fast | HiScript IV All-in-One RT Red SuperMix for qPCR (+gDNA Wiper)Color |
Cat.No. | #R412 | #R423 | #R433 | #RT433 |
Format | 3 tubes | 1 tube | 1 tube | 1 tube |
Kit contents | 5 × gDNA wiper Mix 4 × HiScript IV RT SuperMix Oligo (dT)20VN Random Primers 4 × No RT Control Mix RNase-free ddH2O | 5 × gDNA wiper Mix 4 × HiScript IV qRT SuperMix 4 × No RT Control Mix RNase-free ddH2O | 4 × All-in-one Ultra qRT SuperMix RNase-free ddH2O | 4 × HiScript IV All-in-One qRT Red SuperMix RNase-free ddH2O |
Optimal reaction temperature | PCR: 50℃ qPCR: 37℃-50℃ | 37℃-50℃ | 50℃ | 50℃ |
Application | Flexibility in reaction conditions | First-strand cDNA synthesis reaction mix for two step RT-qPCR | Complete gDNA elimination and Reverse transcription in only 1 step | For customers who subsequently use 384-well plates for qPCR or require color-based tracing. |
