Product Description
VeZol-Pure Total RNA Isolation Kit is applicable for isolating high-quality Total RNA from various samples such as cultured cells, animal and plant tissues. This kit combines phenol/guanidine-based lysis of samples with silica membrane-based purification of total RNA, with the advantages of strong lysing ability, wide compatibility, fast, high yields, and good purity. Samples are fully lysed with VeZol Reagent,and RNA is specifically adsorbed from the aqueous phase by silica membrane. The obtained total RNA can be directly used for molecular biology experiments such as RT-PCR, qRT-PCR, Northern Blot, Dot Blot, in vitro translation, and high-throughput sequencing.
Features
Higher yields and better purity: The yield of RNA obtained is higher than that obtained by spin column purification. The purity of RNA obtained is higher than that obtained by TRIzol.
Super lysis ability: It is effectively isolated from a variety of sample sources (cells, animal tissues, fresh blood, and plant tissues).
Easy and fast: The entire procedure typically takes 20 minutes.
Performance
Wide Compatibility
Different animal tissues that are rich in RNA (e.g., liver,heart)or low in RNA (e.g.,adipose) , cells, simple plants, fresh whole blood were extracted. The results are shown in Figure 1, which incidates Vazyme #RC202 shows wide compatibility.
Figure 1. Gel image of RNA products from different samples
High Quality
RNA from 30 mg rat liver, 1×106 A549 cells, 50 mg rice leaves were extracted with Vazyme #RC202 and other similiar products supplier A-D. The data show RNA products have good quality.
Figure 2. Comparison test of RNA yields and integrity
Figure 3. Comparison test of RNA purity
Components
Storage
Store BOX 1 at 2 ~ 8°C and protect from light. Transport at room temperature.
Store BOX 2 at 15 ~ 25°C and transport at room temperature.
FAQ
Q1:How to increase the yield of RC202 for samples with low RNA content?
A1: You can extend the lysis time appropriately to ensure that the samples are well lysed, or increase the amount of alcohol, from 0.5 × absolute ethanol to 1 x absolute ethanol on the column.
Q2: What is the minimum amount of RNA elution volume?
A2: The manual suggests that the elution volume should be 50-200 μl. If the RNA content of the sample is low, the elution volume can be reduced appropriately, but it is suggested that it should be not less than 20 μl.
Q3: Can RC202 be operated at room temperature?
A3: RC202 can be operated at room temperature except for the lysis and chloroform centrifugal extraction steps, which must be centrifuged at 4℃.
Q4: Precautions for extracting blood samples.
A4: Currently blood samples are recommended to be extracted from fresh blood samples, and it is recommended that 0.9 ml VeZol Reagent be used for 0.1 ml samples. Too much input may result in protein and genomic residues.
Q5:In which steps can RC202 be suspended for preservation?
A5: After the sample is added to VeZol Reagent or chloroform: it can be stored at -80℃, -20℃ for one month and 4℃ for two weeks; after the supernatant is taken: it can be stored at -80℃, -20℃ and 4℃ for one month.
Q6: Can RC202 extract viral RNA?
A6: Yes.
