Product Description
The FastPure Cell/Tissue Total RNA Isolation Kit V2 provides a rapid method for extracting total RNA from animal tissues and cells. The kit is based on silica gel membrane purification technology and does not require β-mercaptoethanol, phenol/chloroform, or any other toxic reagent during the extraction process. It takes only 6 min to extract high-quality RNA. The kit contains FastPure gDNA-Filter Columns III which can effectively remove impurities and gDNA. FastPure RNA Columns III can efficiently bind RNA with an optimized buffer to obtain high-purity total RNA. The isolated RNA has little gDNA residues and no proteins or other impurities contamination. It can be used for RT-PCR, Real-Time PCR, Microarrarys and other downstream experiments.
Features
Fast: It only requires 6 min at room temperature.
Safer: No need for β-mercaptoethanol and phenol or chloroform.
Minimal gDNA residue: No need for on-membrane digestion with DNase I, one-step gDNA contamination removal.
High RNA purity: High purity of extracted RNA, which is ready for use in demanding downstream experiments.
Widely applicable: Compatible with all kinds of cell and tissue samples.
Performance
Super Fast
Obtain one samples in just 6 min at room temperature.
High Quality
Vazyme #RC112 ('V' in the following figures) and commercially available related products (from suppliers A, B, C, D, E ,F and G respectively) were used to extract total RNA from the rat liver tissue (10 mg) and HEK 293 cell (1×106) samples according to the respective Instructions for Use. Figure A is the agarose gel electrophoretogram of the extracted RNA, while Figures B and C are the statistical charts of RNA concentration and purity results. As shown in the figures, RNA extracted by Vazyme #RC112 has good integrity, high yield and high purity.
Wide Sample Compatibility
Vazyme #RC112 was used for the RNA extraction and purification of different samples, and the final RNA product was detected by agarose gel electrophoresis. It shows that Vazyme #RC112 is well compatible in the extraction of RNA from different cell and animal tissue samples.
Here shows some data from various samples.
Note: Typical yields can vary due to factors such as species, developmental stage, and growth conditions.
Components
Storage
Store at 15 ~ 25°C and transport at room temperature.
FAQS
Frequently Asked QuestionsWhat is the maximum binding capacity of the RNA absorbtion column in the RC112 kit?200 μg
What's the gDNA column removal efficiency15μg DNA inputs can be removed with over 99.5% efficiency.
If want to use DNase I to remove DNA, whats the solution?Vazyme provides DNase I set, protocol of RH104-C1 can be followed.
How to improve RNA yields?● For samples with high fiber content, such as muscle, to obtain higher yields, we recommend the following extraction procedure: add 300 μl Buffer RL to approximately 10 mg of tissue, vortex to mix, then add 590 μl RNase-Free ddH2O and 10 μl Proteinase K (Vazyme #DE102), mix and incubate at 56°C for 10 - 20 min. The subsequent steps follow section 08-2/RNA Extraction of the manual. ● For insect samples, follow the 08-2/RNA Extraction/step 2 for liver, add 1 volume of 50% ethanol instead of 0.5 volume of 100% ethanol. ● For bacterial samples, we recommend using Bacteria RNA Enhancement Reagent (Vazyme #R412-C1) to get better recovery. The specific operation is as follows: collect the pellets from 1 - 1.5 ml of bacterial culture by centrifugation, add 200 μl of auxiliary agent preheated at 95°C to the pellets, resuspend the bacteria and incubate at 95°C for 4 min (it is not recommended to exceed 5 min), then add 300 μl Buffer RL and mix well. The subsequent steps follow section 08-2/RNA Extraction of the manual. ● For samples with high RNase content, such as pancreas, spleen, small intestine, etc., it is recommended to add 1% β-mercaptoethanol to the Buffer RL, and then proceed according to the manual.
