Product Description
EGFP mRNA expressed enhanced green fluorescent protein (enhanced green fuorescent protein, EGFP), which was initially isolated from jellyfish (Aequorea victoria) with a maximum emission wavelength of 509 nm, and the excited green fluorescence could be easily detected by fluorescence microscopy or flow cytometry. As a reporting mRNA, EGFP mRNA is ideal for monitoring and optimizing transfection efficiency and can be used as a positive control for mRNA transfection.
This mRNA contains high level of Cap1 structure at 5' terminal .Compared with the Cap 0 structure, the mRNA with the Cap 1 structure is more suitable for the mammalian system and has a higher transcription efficiency. Modified UTP and addition of poly(A) tails provide higher transcriptional efficiency and inhibit RNA-mediated innate immune activation while enhancing mRNA stability and longevity in vitro and in vivo.
Features
Ready-to-use & Time-saving: Eliminates time-consuming mRNA preparation—ready for immediate transfection or LNP formulation.
High capping efficiency: Features Cap 1 structure, optimized for enhanced compatibility in mammalian systems.
Incorporation of modified nucleotides and poly(A) tail: Mitigates RNA-induced innate immune activation and improves mRNA stability.
High transfection efficiency & Excellent reproducibility: Ensures consistent and reliable experimental results.
Performance
Fluorescence images and flow cytometry plots of Vazyme EGFP mRNA expression in HEK 293T cells 20 hours after transfection.
Components
Storage
Store at -30~-15℃ and transport at ≤0℃.
FAQS
Frequently Asked Questions1.Q：Are there any recommended delivery systems?A：For early-stage in vitro screening, we recommend using Trans-IT® or Lipofectamine™ 3000 for RNA transfection. For in vivo delivery systems, there are currently no specific vendor recommendations available.
2.Q：How long can the mRNA stability be maintained after LNP encapsulation?A：The stability duration varies depending on the LNP formulation. It is recommended to determine stability experimentally by measuring mRNA integrity (e.g., via fluorescence-based assays) or protein expression levels in cells.
3. Q: Can you provide the specific sequence of the mRNA?A:The sequence of the reporter gene mRNA is proprietary and confidential R&D information and is not available for public disclosure.
