Product Description
Mut Express Universal Fast Mutagenesis Kit is based on ClonExpress homologous recombination technology, capable of introducing site-directed mutations to 1 - 5 discontinuous sites on the target plasmid simultaneously in a single operation. This kit integrates the amplification module of 2 × Phanta Flash Master Mix and rapid cloning module of 2 × CE Mix V3. The 2 × Phanta Flash Master Mix features high fidelity and rapid amplification characteristics, and it is a one-tube mix, requiring only the addition of primer and template for amplification. When performing site-directed mutagenesis using the Mut Express Universal site-directed mutagenesis kit, the primer design is flexible, and the Input amounts of template are extremely low, which facilitates the complete degradation of the original methylated template. The 2 × CE Mix V3 can accommodate 1 - 5 site-directed mutations. If the amplification products are specific, the Dpn I digestion products can be used directly for recombination reactions without DNA purification, making it the preferred kit for DNA multi-site mutations.
Features
Universal system: Compatible with 1-5 mutation sites
High efficiency: A single-site mutation on a 10 kb plasmid can be completed in just 1.5 h
Simple operation: Amplified product can be directly used in the recombination reaction after being digested with DpnI
Widely applicable: suitable for amplification of any site in the plasmid within 20 kb
Components
Storage
Store at -30 ~ -15°C and ship at ≤0°C.
