Product Description
miRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a specific kit for stem-loop miRNA cDNA one-strand synthesis. It includes a genomic DNA removal step, which can quickly remove the contamination of genomic DNA at 42°C for 2 min. This ensures that subsequent results are more reliable. The HiScript II Reverse Transcriptase on which the Kit is based has extremely high thermal stability, and with an optimized buffer system, it is beneficial to the generation of miRNA-specific reverse transcriptase products. For subsequent quantification of cDNA products, Vazyme's miRNA Universal SYBR qPCR Master Mix (Vazyme #MQ101) is recommended to obtain optimal experimental results.
Features
Excellent linear relationship: It has a good linear relationship in a wide range of template input and can detect pg-level RNA templates.
Superior reaction system: The optimized Buffer composition and concentration are more suitable for the reverse transcription of microRNA.
Software support: Provide online primer design software 'miRNA Primer Design'.
Components
Storage
Store at -30 ~ -15°C and transport at ≤0°C.
