Product Description
EasyAna dsRNA (Modified) Quantitative Detection Kit (ELISA) 2.0 is developed based on a double-antibody sandwich ELISA platform to quantitatively detect the amount of dsRNA in the IVT product or synthesized mRNA solution.
Features
Easy-to-operate: 1.Pre-coated ELISA Plates can slash over 20 hours* of coating time and minimize the risk of inaccuracies caused by human handling. 2.All components for the experiment are fully equipped
Nucleic acid antibody screening techniques: Antibodies compatible with different dRNA sequences
Excellent performance:
Performance
Dilution accuracy
To evaluate the consistency of the test results of samples gradiently diluted to different concentration. The coefficient variation (CV) between the recalculated concentration and the theoretical concentration after different multiples of dilution of 300 ng/ml dsRNA is less than 20%.
High specificity
We verified that the ssRNA, dsDNA, ssDNA, and the in vitro transcriptional enzymes used during mRNA preparation did not interfere with the detection system within the linear range of 0.046–1.5 ng/ml.
Inter-lot consistency
High, medium, and low-concentration samples were tested with 3 different lots, 10 times per lot, and the CV of lot-to-lot is ＜15%.
Components
Related Products
Pruduct Name | Catalog Numbe |
dsRNA-unmodified Standard (High Concentration) | |
dsRNA-Pseudouridine-U (Standard) | |
dsRNA-N1-me-Pseudo-U (Standard) | |
Concentrated wash buffer(20x) |
Click on the catalog number to be redirected to the product detail page
Storage
Store at 2 ~ 8℃ and protect from direct bright light.
FAQS
Frequently Asked QuestionsQ1: How are the standards synthesized?IVT was chosen to synthesize the positive and negative strands respectively, which were then annealed to form dsRNA, and finally digested by single-stranded RNAase and DNase I to remove the single-stranded RNA (ssRNA) or the template to obtain dsRNA antigen or the standards.
Q2: Is the standard in the kit modified?Yes, modified by N1-Me-Pseudo UTP
Q3: What is the size of the dsRNA standard?500 bp
Q4: Can the kit detect UTP-modified samples?Yes, the Kit exhibited minimal selectivity towards both UTP and N1-ψ modifications as well as dsRNA with different sequences.
Q5: What is the size of the dsRNA recognize?The Kit could recognize dsRNAs larger than 40 bp.
Q6: Which antibodies clones are used (J2/K2/K1 or proprietary) ?The antibodies used in the ELISA reagent kit are company-selected. The capture antibody M2 (mouse IgG2a monoclonal antibody) is used for coating the plate, and the detection antibody is M5 (purified mouse IgM monoclonal antibody).
