Product Description
The product BspQI is a recombinant protein encoded by the BspQI gene of Bacillus sphaericus expressed in E. coli. It is a Type IIS Restriction Enzyme, and its recognition sequence is shown below:
5'······GCTCTTC(N)··············3'
3'······CGAGAAG(NNNN)······5'
This product is a GMP Grade recombinant BspQI, with strict control of host protein, exogenous DNA, RNase and other process-related impurities, as well as microbiological limits and bacterial endotoxins during the production process. The entire production process does not use or add ampicillin or any raw materials and auxiliary materials of animal origin, and adopts the production and quality management standards in accordance with GMP norms to guarantee the traceability of the production process and raw materials and auxiliary materials, and the product meets the requirements for raw materials and auxiliary materials in the field of mRNA vaccine production and other fields.
Features
Generates intact full-length poly(A) tails with Type IIS precision
Compatible with diverse plasmid workflows and residual matrices
Fast and efficient, complete digestion in 1 hour
Performance
Components
Storage
Store at -20 ± 5°C and transport at ≤0°C.
FAQS
Frequently Asked QuestionsQ1. What should be noted during digestion?Key recommendations: 1.Keep enzyme volume ≤10% of the total reaction volume. 2.Heat inactivation: 80°C for 20 min. 3.Assess plasmid linearization by agarose gel electrophoresis, HPLC, or CE. 4.Use phenol-chloroform extraction if RNase removal is required.
Q2. What may cause incomplete plasmid digestion?Possible causes and solutions: 1.Suboptimal reaction conditions: Optimize enzyme-to-DNA ratio or extend digestion time. 2.DNA contamination: Replace high-salt buffers with RNase-free water; remove impurities using phenol-chloroform extraction. 3.Enzyme inactivation: Store at -20°C, aliquot before use, and avoid repeated freeze-thaw cycles. 4.Improper enzyme dilution: Use storage buffer for dilution. 5.Missing recognition site: Confirm plasmid sequence by Sanger sequencing. 6.High open-circle content: Optimize plasmid prep to increase supercoiled DNA (avoid vigorous mixing). 7.Plasmid multimers: Use an appropriate host strain or increase enzyme amount.
Q3. Why do smeared bands appear after digestion?Possible causes and solutions: 1.Poor DNA quality: Use suitable host strains or perform phenol-chloroform extraction. 2.Reagent contamination: Use fresh running buffer and clean agarose gels. 3.Excess enzyme: Reduce enzyme amount and re-optimize conditions.
