Product Description
This kit extracts high-purity plasmid DNA from 1 - 4 ml of overnight cultured bacterial cultures, utilising an optimized alkaline lysis method, which improves the efficiency of nucleic acid release. A unique silica matrix membrane adsorption technology is emplyed to bind plasmids effectively while removing proteins, polysaccharides, and other impurities. A unique indicator-containing P2 solution provides visual confirmation of complete lysis and neutralization, ensuring extraction quality and facilitating visual operation. The yield of extracted plasmid DNA is high, requiring only 8 minutes, and the purified plasmid DNA can be directly used for restriction endonuclease digestion, PCR, sequencing, ligation, conversion, transfection of certain conventional cell lines, and other biological experiments.
Features
Rapid processing: Complete extraction in 8 min
Excellent performance: Obtain up to 25 μg of high purity plasmid DNA
Visualization: Easier supernatant aspiration with color indication
Experimental Cases
Rapid processing: Complete extraction in 8 min
Good quality
Sample | Input amount | Yield | Sample | Input amount | Yield |
pGBKT7 | 4 ml | 19.0 μg | pCDNA3.1(+) | 4 ml | 25.8 μg |
pET28a | 4 ml | 4.5 μg | pACT-eGFP | 4 ml | 19.1 μg |
pTT5 | 4 ml | 16.2 μg | L4440 | 4 ml | 5.9 μg |
pUC5-7 | 4 ml | 14.5 μg | pLV[Exp]-Puro-CMV>EGFP | 4 ml | 15.0 μg |
Bds106 | 4 ml | 11.5 μg | pGKJE8 | 4 ml | 4.1 μg |
17K | 4 ml | 17.1 μg | pBlueScriptII-SK(+) | 4 ml | 16.5 μg |
Convenient operation
Visual color confirmation indicates adequate lyse neutralization, and the compact flocculent precipitate enabling easier supernatant aspiration.
Components
Storage
Store at 15 ~ 25°C and ship at room temperature.
