The kit is intended for fast and easy genomic DNA (gDNA) isolation from 50 μl – 1 ml of fresh or frozen anticoagulated whole blood samples. This kit utilizes superparamagnetic particle paried with unique reagents for the effective removal of various impurities and specific adsorption of gDNA. The workflow does not require organic reagents such as phenol or chloroform, and highly purified gDNA can be obtained reproducibly within 1 h. The extracted gDNA can be directly used for downstream experiments, such as enzyme digestion, PCR, qPCR, library preparation, and Southern blot.