The VAHTS Target Capture Core Exome Panel is designed with Imwaldt probes and consists of over 400,000 120 nt ssDNA probes. Human GRC h38/hg38 was used as the reference genome, covering 19,441 genes in Refseq, CCDS, ClinVar and other databases, and the target capture region was 33.9 Mb, which was compatible with mixed capture of up to 12 samples.