HiScript II Reverse Transcriptase is a novel reverse transcriptase obtained by in vitro molecular evolution technology, based on M-MLV (RNase H-) Reverse Transcriptase, HiScript II Reverse Transcriptase greatly improves the thermal stability and cDNA synthesis efficiency. The HiScript II Q RT SuperMix for qPCR (+gDNA wiper) is specially designed for two-step qRT-PCR. The residual genomic DNA in RNA template can be removed rapidly with the 4 × gDNA Wiper Mix. The 5 × HiScript qRT SuperMix II contains all necessary components required for reverse transcription. With the addition of template RNA and ddH ₂ O, the reaction can proceed quickly, meanwhile, gDNA wiper is terminated to ensure the integrity of the cDNA.
This product has been specially optimized for qPCR. With the optimized proportion of Random primers/Oligo (dT) ₂₃ VN primer mix, cDNA synthesis can start from each region of RNA transcript and have the same reverse transcription efficiency, which ensures the authenticity and reproducibility of qPCR results. The RT product is compatible with dye-based and probe-based qPCR, and can be used with the corresponding reagent according to the experimental purpose to perform high-performance gene expression analysis.