HiScript II One Step qRT-PCR Probe Kit is specially designed for qPCR detection using RNA (e.g., RNA virus) as templates. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube. No additional opening/pipetting operations are required, greatly increasing assay throughput and reducing the risk of contamination. Combining the superior performance of HiScript II Reverse Transcriptase and hot-start Champagne Taq DNA Polymerase, equipping with an optimized buffer, the detection sensitivity of HiScript II One Step qRT-PCR Probe Kit can reach 0.1 pg of total RNA or <10 copies of RNA templates. This product is provided as mastermix. The 2 × One Step Mix contains an optimized buffer and dNTP mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g., TaqMan).