AceTaq® DNA Polymerase is a chemically modified Taq DNA Polymerase whose activity is completely blocked at room temperature to prevent non-specific amplification and primer dimers during sample preparation and reaction temperature rise. The activity is released only after heating at 95°C. Compared with antibody-based hot-start Taq enzymes, AceTaq® DNA Polymerase has a more complete and stricter activity closure; compared with existing chemically modified hot-start Taq enzymes, the activation time of AceTaq® DNA Polymerase only takes 5 minutes. Compatible with existing PCR programs. AceTaq® DNA Polymerase combined with an optimized buffer system can minimize non-specific amplification and primer dimers, bring the highest sensitivity, and is very suitable for amplifying low-copy genes from complex templates (genome, cDNA). The PCR product has an A at the 3’ end, which can be cloned into the T vector and is suitable for ClonExpress® Rapid Cloning Kit (C112-C113).