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- Purmorphamine | Hedgehog signaling activator
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- Purmorphamine | Hedgehog signaling activator
Product Description
Purmorphamine, a purine-based agonist of the Hedgehog signaling pathway, selectively induces osteogenesis in multipotent mesenchymal progenitor cells. In addition, purmorphamine regulates downstream targets such as Gli1 and Patched, which led to the discovery of its direct effect on Smoothened. [1, 3] Purmorphamine acts in contrast to known Hedgehog antagonists, such as cyclopamine and forskolin. In a luciferase-based assay, the EC50 of purmorphamine was found to be 0.5 uM. [1]
In an independent study on C3H10T1/2 cells, the EC50 of purmorphamine was found to be 1 uM, inducing a 50-fold increase in alkaline phosphatase (ALP). [2, 3]
Technical information:
| Chemical Formula: | C31H32N6O2 | |
| CAS #: | 483367-10-8 | |
| Molecular Weight: | 520.62 | |
| Purity: | >98% | |
| Appearance: | White | |
| Chemical Name: | 9H-Purin-6-amine, 9-cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)- | |
| Solubility: | Up to 22 mM in DMSO | |
| Synonyms: | Purmorphamine |
Shipping Condition: The product is shipped in a glass vial at ambient temperature.
Storage condition: For longer shelf life, store solid powder at 4oC desiccated, or store DMSO solution at -20oC.
Reference:
| 1. | Wu et al., Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem. Biol. 2004, 11, 1229-1238. Pubmed ID: 15380183 |
| 2. | Wu et al., A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells. J. Am. Chem. Soc., 2002, 124, 14520-14521. Pubmed ID: 12465946 |
| 3. | Sinha et al., Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat. Chem. Biol. 2006, 2(1), 29-30. Pubmed ID: 16408088 |
Other Information:
Product Specification (pdf)
MSDS (pdf)
Certificate of Analysis is available upon request.
